Allow for inoculum to generally be thoroughly absorbed just before incubating for 24 h at thirty°C. Check out plates for lecithinase output as indicated by zone of precipitation encompassing progress. Mannitol isn't fermented by isolate if development and surrounding medium are eosin pink. (Yellow shade suggests that acid is developed from mannitol.) B. cereus colonies are generally lecithinase-good and mannitol-negative on MYP agar.
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